Transcriptomic and metabonomic profiling of obesityprone. Using dietinduced obesity to understand a metabolic. In a hypercaloric diet model with fat, a diet was used containing 17. Rat models of dietinduced obesity and high fatlow dose. Current study comprises usage of a diet induced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. When compared over 10 wk of free feeding, hf rats weighed 10% more p. Pdf modified highsucrose dietinduced abdominally obese and. Diet induced obese dio mouse models c57bl6ntac male. Effect of high fatdiet and obesity on gastrointestinal. The rats observed were those in the authors own rattery.
A humanized rat model of prediabetes by high fat diet. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Oxidative stress biomarkers and histology in highfat. In conclusion, administration of leptin to pregnant rats during the third trimester of pregnancy and lactation reduced susceptibility to diet induced obesity, glucose intolerance, and insulin resistance in their offspring, especially the females. Obesity can be induced in animals by neuroendocrine, dietary or genetic changes. Effect of streptozotocin on glucose levels in albino wister rats. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. In dietinduced obese male mice decreases in sperm motility 16,17. It is assumed that honey would not reduce the level of hyperglycaemia induced by fructose, even if it does in the rats with alloxaninduced dm. Selective inbreeding for obesity and diabetes resulted in a strain that exhibits these characteristics without the lepr fa mutation. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. The jackson laboratory maintains an inventory of dietinduced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them.
We observed significant differences in transcriptional expression of nearly 80 genes, some of which are known to be involved in lipid metabolism, transport, and. C57bl6ntac males are put on special diet at 6 weeks of age and. Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type ii diabetes, hypertension and cancer. Obesity is inflammatory disease, rat study shows medicine. Taconic biosciences maintains an inventory of c57bl6ntac male mice on 60% kcal high fat irradiated diet. Another author, studying gastric bypass models in spraguedawley rats, induced obesity with food consisting of 48% chow, 8% maize oil and 44% condensed milk for 7 to 12 weeks 48.
Effect of streptozotocin on glucose levels in albino. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. This study characterizes the highfat diet hfdfed rats as a model for prediabetes or impaired glucose tolerance showing clinical presentation and pathophysiology of natural history of prediabetes in human. Dietinduced obesity in rats leads to a decrease in sperm. The present study used spraguedawley rats that, when fed a highfat diet, exhibit either an obesity prone diop or obesity resistant dior phenotype, to determine whether changes in gut epithelial function and microbiota are diet or obese associated. We hypothesized that excess fuel supply in a rat model of dietinduced obesity leads to myocardial cell damage due to increased oxidative stress and apoptosis. Rodents respond to chronic high fat diet in at least two ways. Accumulating evidence suggests that maternal obesity may increase the risk of metabolic disease in the offspring. Modeling dietinduced obesity with obesityprone rats ncbi. Guidelines to increase the effectiveness of the dietinduced obesity model for the model of dietinduced obesity to be e ective, some measures related to the environment where the animal is kept must be observed, especially when rodents without genetic mutations are studied, such as wistar rats.
Highfat diet increases pain behaviors in rats with or. However, transgenic rats do exist for research purposes. According to the data there are dietary, genetic and pharmacological models. About 15% of the rats refused to run on a treadmill and were excluded from the study at the end of the preliminary stage.
Nov 24, 2016 obesityprone op rats were maintained on a hf diet for 16 weeks to induce obesity. This dietary induced obesity dio prone rat model of obesity is a outbred, albino, and polygenic model that has been selectively bred to develop obesity associated with impaired glucose tolerance, dysplipidemia, and insulin resistance when fed a highfat, highsucrose, and highcaloric diet. Emulsifiers in processed foods may lead to increased risk. Guidelines to increase the effectiveness of the diet induced obesity model for the model of diet induced obesity to be e ective, some measures related to the environment where the animal is kept must be observed, especially when rodents without genetic mutations are studied, such as wistar rats. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear. Diet induced obese dio mouse models c57bl6ntac male mice. The obesity was manifest as a modest but nonetheless significant 10% increase in total body weight.
Prenatal influences on leptin sensitivity and susceptibility to dietinduced obesity. Biometric evidence of dietinduced obesity in lewcrl rats article pdf available in comparative medicine 612. Herzog yz y human performance laboratory, university of calgary, ab, canada z mccaig institute for bone and joint health, university of calgary, ab, canada. Rats with blood glucose over 160 mgdl were considered as.
Highfat diet consumption for 8 weeks affected body weight in longevans but not spraguedawley rats. Introduction over the last decades, obesity has become a global epidemic and an important public health problem in many countries. To date, animal models of obesity based on gene modi. Diet induced obese rats weighed significantly more than control rats after 24 weeks on the high fat diet. After 4 weeks on either diet, caf rats weighed significantly more than c rats 278. Young wistar rats 4050 g were fed a hfd 45% energy from fat or a normal diet for 6 month. Transcriptomic and metabonomic profiling of op and or rats has been conducted, showing two sets of significantly different phenotypic profiles in response to 16 weeks of high fat diet. We determined the anti obesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of hfd induced obesity for 10 weeks.
Rats were calorically restricted on an lf diet to induce 1015% loss, maintained at this reduced weight for 0, 8, or 16 weeks, then allowed to relapse with free access to lf diet. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. Effect of high fatdiet and obesity on gastrointestinal motility. We investigated the effects of established maternal dietinduced obesity on male and female offspring appetite, glucose homeostasis and body composition in rats. Obesity and metabolic syndrome are associated with glomerulosclerosis and proteinuria, but the mechanisms are not known. Greater dietinduced obesity in rats consuming sugar solution. Current study comprises usage of a dietinduced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. Established dietinduced obesity in female rats leads to.
This allows researchers to study a specific condition, such as obesity, without being effected by the genetic difference. Only male zdsd rats develop diabetes hyperglycemia, 500 mgdl and some convert between 15 and 21 weeks of age. Modeling dietinduced obesity with obesityprone rats. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. In conclusion, a sucroserich diet induced obesity, insulin resistance, oxidative stress, and nafld in rats. Dietinduced obesity in c57bl6j mice causes increased. As mentioned in table 3, it has been observed that rats feed on highfat diet consecutively for 21 days resulted in a marked increase in the level of lipids, characterized by elevated levels of total cholesterol, triglycerides, ldl, vldl, and reduced levels of hdl when compared to normal control, that is, rats receiving the normal feed. Energy expenditure in rats with dietinduced obesity article pdf available in american journal of clinical nutrition 442. Dietinduced obesity in c57bl6j mice causes increased renal. In stapled rats, segment weight and mucosal weight were lower in the excluded segment, and higher in the transposed segment, compared to all other rats. Leptin programmed both reduced intake of the obesogenic diets and increased energy expenditure. Obesity and adipocyte abnormalities in offspring of rats undernourished during pregnancy. Pdf biometric evidence of dietinduced obesity in lew. A controlled highfat diet induces an obese syndrome in rats.
This study was designed to investigate the antiobesity activities of ethanolic extract of terminalia paniculata bark tpee on high fat dietinduced obese rats. Dietinduced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Research article rat models of dietinduced obesity and. Type i dm was induced in 20 rats by a double ip injection of stz sigma as 30 mg kg for two consecutive days with a total of 60 mg kg rat.
May 01, 2017 young animals have a different metabolism and accumulate less adipose tissue due to the metabolic characteristics appropriate to the age bayol and collaboradors, 2010, it also being advisable that older animalsof approximately 100 days oldshould be subjected to diet induced obesity rolls, van duijvenvoorde and rowe, 1983. Rats were fed the same hf purified diet 46% kcal fat. Ii in a diet induced obesity state, dio rats show insensitivity to the damphetamine effect on icss, but show equal sensitivity. Western diet induces apoptosis in the heart of a rat model. Effects of the application of a highfatcarbohydrate diet. Jul 15, 2015 however, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far.
In the past there has been much debate, and research, over the relationship between type 2. Propensity to highfat dietinduced obesity in rats is. Typically mice, rats, dogs, or nonhuman primates are used in these models. It is well established that increased availability and utilization of free fatty. In the obese rats, a reduction in nhe3 expression in the proximal tubule may impact on the acidification of endosomes which are responsible for albumin uptake, suggesting a key role for the exchanger in protein endocytosis in obesity. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. In this study, we used the paradigm of diet induced obesity first described by woods et al. The induced obesity resulted from adipocyte enlargement alone, early studies of the development of adipose tissue cellularity in rats and mice using cellcounting tech. In this part of the study rats were given a highfat diet. In this study, we used the paradigm of dietinduced obesity first described by woods et al. Thus, we tried to understand the effects of obesity on adult female liver. A new rat model zdsd of type 2 diabetes independent of.
In a first experiment, male wistar rats were fed a highfat diet hfd. The model of diet induced obesity in rodents can be considered effective when the objective is the study of the physiopathology of metabolic and vascular complications associated with obesity. Dietinduced obesity, adipose inflammation, and metabolic dysfunction correlating with par2 expression are attenuated by par2 antagonism. Wistar and sd rats were assigned for 2 experimental groups for 17 weeks. Rats with free access to an hf diet were fed more energy and became obese relative to rats with free access to a diet containing the same constituents but less fat. Pdf energy expenditure in rats with dietinduced obesity.
Methods female wistar rats were fed either a standard chow 3% fat, 7% sugar wtwt or a palatable obesogenic diet 11% fat, 43% sugar wtwt for 8 weeks before mating and throughout pregnancy and lactation. Physiogenex preclinical cro for the evaluation of your drugs efficacy in metabolic diseases keywords insulin resistance, glucose intolerance liver steatosis, hyperinsulinemic euglycemic clamp, glucoses fluxes, diabetes, pharmacology, in vivo. Therefore, the dietinduced obese spraguedawley rat may serve as a. Thus, the aim of the present study was to investigate the effect of highfat diet induced obesity on male reproductive parameters. The development of hypertension in a rat model of diet induced obesity is described in the literature. Research article a novel wistar rat model of obesityrelated. The aim of this study was to examine the anti obesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Transcriptomic and metabonomic profiling of op and or rats has been conducted, showing two sets of significantly different phenotypic profiles in response to 16 weeks of high. The present study used spraguedawley rats that, when fed a highfat diet, exhibit either an obesityprone diop or obesityresistant dior phenotype, to determine whether changes in gut epithelial function and microbiota are diet or obese associated. May 17, 2016 preventing high fat diet induced obesity and improving insulin sensitivity through neuregulin 4 gene transfer.
The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of dietinduced obesity. Dietinduced adipocyte number increase in adult rats. Treating diet induced obese rats with enalapril or placing them on a normal diet for 12 weeks, after 12 weeks of the high fat diet, resulted in diet induced obese rats weighing about the same as control rats at the end of the study. Preventing high fat dietinduced obesity and improving insulin sensitivity through neuregulin 4 gene transfer. Controlled highfat diet induces an obese syndrome in rats. Young animals have a different metabolism and accumulate less adipose tissue due to the metabolic characteristics appropriate to the age bayol and collaboradors, 2010, it also being advisable that older animalsof approximately 100 days oldshould be subjected to dietinduced obesity rolls, van duijvenvoorde and rowe, 1983. Mar 14, 2009 accumulating evidence suggests that maternal obesity may increase the risk of metabolic disease in the offspring. Using dietinduced obesity to understand a metabolic subtype. C57bl6ntac males are put on special diet at 6 weeks of. Mar 11, 2011 obesity is rapidly becoming a worldwide epidemic that affects children and adults. Using dietinduced obesity to understand a metabolic subtype of osteoarthritis in rats k. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of diet induced obesity.
Antiobesity effects of boiled tuna extract in mice with. Antiobesity and hypolipidemic activity of moringa oleifera. Differential effect of honey on selected variables in. Diet induced obesity, adipose inflammation, and metabolic dysfunction correlating with par2 expression are attenuated by par2 antagonism. Protective effects of orlistat on lipid profile, cardiac. Dietinduced obesity in rats leads to a decrease in. The zucker rat is often used in studies involving obesity. The prediabetic weight gain of the zdsd rat is similar to zf and zdf rats. Evaluation of antiobesity activities of ethanolic extract of. Preventing high fat dietinduced obesity and improving. Rats are known for their ability to squeeze through small spaces and get into everything. Male wistar rats n8group were fed either low fat, western, or high fat diets 10%, 45%, or 60% calories from fat, respectively for acute 17days, short 12 months, intermediate. Obesity is rapidly becoming a worldwide epidemic that affects children and adults.
Yet, many pet rats sadly cant accomplish this trick, because they are overweight. Using diet induced obesity to understand a metabolic subtype of osteoarthritis in rats k. Prevention of dietinduced obesity and impaired glucose. Ro dent mo del for the study of obesityrelated disor ders rev assoc med bras 2012. Jul 10, 2012 greater diet induced obesity in rats consuming sugar solution during the inactive period. Rats on a cafeteriastyle caf diet 10 had free access to a semisolid diet containing 33% ground commercial rat chow, 33% full fat sweetened condensed milk nestle, 7% sucrose, and 27% water. Rats as a model for obesity research multidisciplinary. Effect of streptozotocin on glucose levels in albino wister rats khaleel nagarchi1, saif ahmed2, anusha sabus3, shaik hussain saheb4. The hypercaloric pellet diet cycle promoted an increase in body weight and fat, systolic. Its amazing the narrow cracks and crevices that lithe little rats manage to get through. Taconic uses research diets industry standard high fat rodent diet, d12492. In the past there has been much debate, and research, over the relationship between type 2 diabetes and obesity.
The induced obesity resulted from adipocyte enlargement alone, early studies of the development of adipose tissue cellularity in. Modified highsucrose dietinduced abdominally obese and normalweight rats developed high plasma free fatty acid and insulin. Wistar and sd rats were assigned for 2 experimental groups for. Thus, the aim of the present study was to investigate the effect of highfat dietinduced obesity on male reproductive parameters. Female wistar rats were fed either a standard chow 3% fat, 7% sugar wtwt or a palatable obesogenic diet 11% fat. Greater dietinduced obesity in rats consuming sugar. We determined the antiobesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of. Postprandial colonic response was studied after a high carbohydrate or high fat meal in normal individuals and both meals caused. We developed a rat model of dietinduced obesity that will be a powerful tool for assessment of this issue. A hypercaloric pelletdiet cycle induces obesity and comorbidities. Data in table 2 demonstrate that feeding rats a high fat diet to induce obesity or subsequent treatment by placing obese rats on a normal diet or treating them with enalapril or menhaden oil enriched diet did not significantly change serum levels of free fatty acids, triglycerides, or cholesterol.
Research article a novel wistar rat model of obesity. We hypothesized that excess fuel supply in a rat model of diet induced obesity leads to myocardial cell damage due to increased oxidative stress and apoptosis. Greater dietinduced obesity in rats consuming sugar solution during the inactive period. Transcriptomic and metabonomic profiling of obesityprone and. The effect of dietinduced obesity on toxicological parameters in. We investigated the effects of established maternal diet induced obesity on male and female offspring appetite, glucose homeostasis and body composition in rats. Treatingdietinducedobeserats with enalapril or placing them on a normaldiet for weeks, a er weeks of the high fat diet, resulted in diet induced obese rats weighing about the same as control rats at the end of the study. The aim of this study was to examine the antiobesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Emulsifiers in processed foods can lead to an increased risk of obesity, metabolic syndrome.
Since rats and mice show a similar relationship, they are considered an appropriate model. Control c rats had free access to a standard nonpurified diet containing 51% carbohydrate, 4% fat, and 21% protein. It is intended to mimic the most common cause of obesity in humans. I before the onset of obesity dio rats show an innate decreased sensitivity to the effects of damphetamine on icss threshold and feeding, as compared to dr rats. Pdf dietinduced obesity in rats leads to a decrease in sperm. Diet induced obesity an overview sciencedirect topics.
1438 1041 794 330 460 412 873 752 1393 1261 695 754 432 131 113 371 840 141 1544 48 11 442 1155 456 696 868 1146 552 1119 709 817 1269 346 349 161 1217 765 725